The conditions you have to meet are specific to the color-coded spaces. For example, if it provides a single number, every side of a tile in that space must add up to the number provided. It is possible – and common – for only half a tile to be within a color-coded space.
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While Sanger sequencing was highly accurate and less labor-intensive than its predecessors, it still required the use of radioactive reagents and manual sequence recording. In 1986, Leroy Hood’s lab at Caltech replaced the radiolabeled ddNTPs with fluorescently labeled nucleotides, using fluorophores that emitted different colors of light for each base. They were now able to run the products of all four reactions on the same gel and have a computer read the sequence by detecting the color of each fluorescent signal as fragments passed through a laser beam.。safew官方版本下载是该领域的重要参考